Officers and staff from Hanley police station are beginning to move into One Smithfield.
Plans to relocate to the neighbouring building were announced in February 2016, with Police and Crime Commissioner Matthew Ellis stated he believed the police station was no longer fit for purpose.
Justice Services, Forensics, Licensing, Force Safeguarding Investigation and Neighbourhood Policing teams are amongst those who will be based in the site.
It is expected to take several weeks for all staff to move over.
The Customer Services Team, who staff the front counter and are the first point of contact when visiting the police in Hanley, is set to take up residence at the end of July.
They will operate from two front desks in the reception area of One Smithfield and will be available to deal with the public from Monday to Thursday 9am to 5pm; Friday 9am to 4.30pm. The building is closed at weekends and Bank Holidays.
Deputy Chief Constable, Nick Baker said: “This is an exciting opportunity for the force and I am delighted the move into One Smithfield is about to get underway.
“Our transformation programme continues as we move into a state-of-the-art shared building which allows police and partners to work even closer to tackle issues affecting our community.
“Neighbourhood policing is the bedrock of policing in Staffordshire and members of the public will still be able to access their local officers in the way they do now – just simply at a neighbouring building.”
Police and Crime Commissioner for Staffordshire, Matthew Ellis, said: “Improving police finances over the last three years has meant we have been able to provide facilities in Stoke-on-Trent which are fit for policing in the 21st century.
“It is about ensuring officers the right tools for the job to deal with the changing nature of crime and bringing specialist services under one roof.
“The public enquiry desk being integrated with other council services means better sharing of information and it will provide a more joined-up service for local people.”
Councillor David Conway, leader of Stoke-on-Trent City Council, said: “We are pleased that Staffordshire Police will shortly be joining us at One Smithfield.
“Times have changed and public services need to work better together to provide the best services and value to taxpayers.
“By housing the council and police under the same roof at One Smithfield we are doing exactly that.
“This will not only save money but allow us to work closely together to improve the services we offer to residents.
“This move makes perfect sense for us, the police and the taxpayer.
“The move will take One Smithfield close to its full occupancy which is fantastic news for Smithfield as a development and the city centre as a whole.
“It is a further sign of the growing confidence there is in the city centre and I look forward to seeing the positive impact it has for our residents.”