One kitten has died and a second is undergoing treatment after being found ill and abandoned in a skip.
They were found by a woman in a skip outside her home in Cornmill Lane in Tutbury, near Burton on 18th October.
They were rushed to a local vets where staff contacted the RSPCA.
Inspector Laura Bryant said: “They were very unwell with cat flu and were terribly underweight.
“The lady who found them said the kittens were unable to open their eyes and had discharge coming from the nose. They were in a very poor condition.
“Sadly, the little male kitten died but his sister is responding to treatment.”
The kittens, aged around five-weeks-old, were not microchipped. The female, now named Medusa, is receiving continued treatment at a local veterinary surgery.
Inspector Bryant added: “These innocent, defenceless babies were dumped and left to die.
“This highlights the needs for more education to ensure potential cat owners understand the commitment, responsibility and costs involved in taking on a pet, as well as the importance of neutering cats.
“There is no excuse for this sort of behaviour as there is always someone, somewhere who will help people if needed.
“There are lots of organisations, such as the RSPCA, Cats Protection and local vets, who offer assistance with the costs of neutering.
“We are completely overrun with cats and kittens in our centres at the moment and more are coming in every day.”