Crewe Alex suffered their first home league defeat of the season going down 1-0 to Cambridge.
The Alex could not follow up the excellent 5-1 win over Chestefield at the weekend.
The only goal came from Jabo Ibehre ten minutes from half time.
The result saw the Alex drop to tenth in the table.
Dave Artell's side are back in action on Saturday when they make the long trip to take on leaders Exeter.